Retinal vessel segmentation and depiction of morphological attributes of retinal blood vessels, such as length, width, tortuosity, branching patterns and angles are utilized for the diagnosis, screening, treatment, and evaluation of various cardiovascular and ophthalmologic diseases such as diabetes, hypertension, arteriosclerosis. Automatic detection and analysis of the vasculature can assist in the implementation of screening programs for diabetic retinopathy and can help find relation of vascular tortuosity with diagnosis of hypertension, and computer-assisted laser surgery.